1CIW
PEANUT LECTIN COMPLEXED WITH N-ACETYLLACTOSAMINE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 129.380, 126.922, 76.065 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.700 |
R-factor | 0.194 * |
Rwork | 0.194 |
R-free | 0.24400 |
Structure solution method | OTHER |
Starting model (for MR) | PDB2PEL.ENT WITHOUT SUGAR AND WATER |
RMSD bond length | 0.017 |
RMSD bond angle | 2.100 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 99.000 |
High resolution limit [Å] | 2.700 |
Rmerge | 0.124 |
Total number of observations | 185971 * |
Number of reflections | 34005 |
Completeness [%] | 97.8 |
Redundancy | 5.46 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
10 | 1 | reservoir | sugar | 1.5-10 (mM) | |
11 | 1 | reservoir | sodium phosphate | 0.05 (M) | |
2 | 1 | drop | sodium phosphate | 0.05 (M) | |
3 | 1 | drop | 0.2 (M) | ||
4 | 1 | drop | sodium azide | 0.02 (%) | |
5 | 1 | drop | sugar | 1.5-10 (mM) | |
6 | 1 | drop | PEG8000 | 12 (%(w/v)) | |
7 | 1 | reservoir | PEG8000 | 40 (%(w/v)) | |
8 | 1 | reservoir | 0.2 (M) | ||
9 | 1 | reservoir | sodium azide | 0.02 (%) |