1CI6
TRANSCRIPTION FACTOR ATF4-C/EBP BETA BZIP HETERODIMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | RIGAKU |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 72.628, 81.152, 35.317 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| Rwork | 0.217 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fos |
| RMSD bond length | 0.005 * |
| RMSD bond angle | 0.920 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.082 * | 0.475 * |
| Total number of observations | 58744 * | |
| Number of reflections | 6800 | |
| <I/σ(I)> | 8.3 | 3.6 |
| Completeness [%] | 99.0 | 99.9 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 15 * | 2.0 M (NH4)2SO4, 100 MM SODIUM CACODYLATE, PH 6.5, 10.0 % DIOXANE AT 15 DEGREES C, VAPOR DIFFUSION, HANGING DROP, temperature 288K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | C/EBP beta-ATF4 heterodimer | 0.3 (mM) | |
| 2 | 1 | drop | ammonium sulfate | 1 (M) | |
| 3 | 1 | drop | sodium cacodylate | 50 (mM) | |
| 4 | 1 | drop | dioxane | 5 (%) | |
| 5 | 1 | reservoir | dioxane | 10 (%) |
