1CI6

TRANSCRIPTION FACTOR ATF4-C/EBP BETA BZIP HETERODIMER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	RIGAKU
Spacegroup name	P 2 2 21
Unit cell lengths	72.628, 81.152, 35.317
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
Rwork	0.217
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fos
RMSD bond length	0.005 *
RMSD bond angle	0.920 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.082 *	0.475 *
Total number of observations	58744 *
Number of reflections	6800
<I/σ(I)>	8.3	3.6
Completeness [%]	99.0	99.9
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	15 *	2.0 M (NH4)2SO4, 100 MM SODIUM CACODYLATE, PH 6.5, 10.0 % DIOXANE AT 15 DEGREES C, VAPOR DIFFUSION, HANGING DROP, temperature 288K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	C/EBP beta-ATF4 heterodimer	0.3 (mM)
2	1	drop	ammonium sulfate	1 (M)
3	1	drop	sodium cacodylate	50 (mM)
4	1	drop	dioxane	5 (%)
5	1	reservoir	dioxane	10 (%)