1CI4

THE CRYSTAL STRUCTURE OF HUMAN BARRIER-TO-AUTOINTEGRATION FACTOR (BAF)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X9B
Synchrotron site	NSLS
Beamline	X9B
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1998-07-27
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9793,0.9789,0.9686
Spacegroup name	P 43 21 2
Unit cell lengths	41.800, 41.800, 214.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.900
Rwork	0.210
R-free	0.26500 *
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.370 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.061 *	0.184 *
Total number of observations	219206 *
Number of reflections	16011
<I/σ(I)>	16
Completeness [%]	99.5	100
Redundancy	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	6.5		DIALYSIS OF PROTEIN AT 8.3 MG/ML IN 20MM TRIS HCL AT PH7.0, 10%(W/V) GLYCEROL, 150MM NACL, 10MM DTT, AND 0.1MM EDTA AGAINST 20MM IMIDAZOLE AT PH 6.5, 80MM NACL, AND 10MM DTT. DIALYSIS DONE AT ROOM TEMPERATURE.(ALL CONCENTRATIONS ARE IN MILLI-MOLAR)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	imidazole	20 (mM)
2	1	1		80 (mM)
3	1	1	dithiothreitol	10 (mM)