1CI3
CYTOCHROME F FROM THE B6F COMPLEX OF PHORMIDIUM LAMINOSUM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 109.600, 109.600, 145.100 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.203 * |
| Rwork | 0.203 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hcz |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.700 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.032 * | 0.128 * |
| Total number of observations | 126348 * | |
| Number of reflections | 26071 | |
| <I/σ(I)> | 42 | 8.5 |
| Completeness [%] | 97.9 | 83.8 |
| Redundancy | 4.8 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.5 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | zinc acetate | 200 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 8-12 (%) |
