1CHQ
SURPRISING LEADS FOR A CHOLERA TOXIN RECEPTOR BINDING ANTAGONIST; CRYSTALLOGRAPHIC STUDIES OF CTB MUTANTS
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994-11-01 |
| Detector | SIEMENS X1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 102.600, 67.390, 98.810 |
| Unit cell angles | 90.00, 131.63, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.100 |
| R-factor | 0.208 * |
| Rwork | 0.208 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.810 |
| Data reduction software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.100 * |
| Rmerge | 0.053 |
| Number of reflections | 28288 |
| Completeness [%] | 96.0 |
| Redundancy | 2.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.4 | pH 6.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sialic acid | 1 (mM) | |
| 2 | 1 | reservoir | cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | HEPES | 10 (mM) | |
| 4 | 1 | reservoir | 200 (mM) | ||
| 5 | 1 | reservoir | PEG1000 | 13 (%) |
