1CHK

STREPTOMYCES N174 CHITOSANASE PH5.5 298K

Experimental procedure

Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1993-09-29
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 1 21 1
Unit cell lengths	56.300, 59.300, 85.400
Unit cell angles	90.00, 96.20, 90.00

Refinement procedure

Resolution	5.000 - 2.400
R-factor	0.1809 *
Rwork	0.181
R-free	0.17480 *
RMSD bond length	0.016 *
RMSD bond angle	3.118 *
Data reduction software	SDMS
Data scaling software	SDMS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rmerge	0.088
Total number of observations	121478 *
Number of reflections	21903
Completeness [%]	98.7 *
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		Marcotte, E., (1993) J.Mol.Biol., 232, 995. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	sodium acetate	20 (mM)
3	1	drop	potassium phosphate	0.5 (M)
4	1	drop	PEG8000	20 (%(w/v))
5	1	reservoir	potassium phosphate	0.5 (M)
6	1	reservoir	PEG8000	20 (%(w/v))