1CGY

SITE DIRECTED MUTATIONS OF THE ACTIVE SITE RESIDUE TYROSINE 195 OF CYCLODEXTRIN GLYXOSYLTRANSFERASE FROM BACILLUS CIRCULANS STRAIN 251 AFFECTING ACTIVITY AND PRODUCT SPECIFICITY

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	120.184, 110.701, 66.207
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.16 *
RMSD bond length	0.015 *
RMSD bond angle	2.430 *
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.000 *
High resolution limit [Å]	2.500 *	2.500 *
Rmerge	0.087 *
Total number of observations	50751 *
Number of reflections	26191 *
Completeness [%]	83.6 *	32.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *