1CGU
CATALYTIC CENTER OF CYCLODEXTRIN GLYCOSYLTRANSFERASE DERIVED FROM X-RAY STRUCTURE ANALYSIS COMBINED WITH SITE-DIRECTED MUTAGENESIS
Experimental procedure
Spacegroup name | P 21 21 21 |
Unit cell lengths | 94.100, 105.000, 113.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.500 |
R-factor | 0.187 |
Rwork | 0.187 |
RMSD bond length | 0.018 |
RMSD bond angle | 3.600 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
High resolution limit [Å] | 2.500 * |
Rmerge | 0.121 * |
Completeness [%] | 76.0 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.7 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein solution | 50 (%(v/v)) | |
2 | 1 | drop | CGTase | 7-15 (mg/ml) | |
3 | 1 | drop | Tris-HCl | 50 (mM) | |
4 | 1 | drop | PEG1500 | 0.5 (%) | |
5 | 1 | reservoir | ammonium sulphate | 0.9-1.0 (M) | |
6 | 1 | reservoir | CGTase | 7-15 (mg/ml) | |
7 | 1 | reservoir | Tris-HCl | 50 (mM) | |
8 | 1 | reservoir | PEG1500 | 0.5 (%) |