1CGQ

MACROPHAGE MIGRATION INHIBITORY FACTOR (MIF) WITH ALANINE INSERTED BETWEEN PRO-1 AND MET-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	133
Collection date	1997-12-01
Spacegroup name	P 21 21 21
Unit cell lengths	67.810, 67.870, 88.770
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.234
R-free	0.27500
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	1.510
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.000	2.000
Rmerge	0.078 *	0.094 *
Total number of observations	115296 *
Number of reflections	27733 *
<I/σ(I)>	6
Completeness [%]	98.2	96.8
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	ammonium sulfate	2.0 (M)
3	1	reservoir	HEPES	0.1 (M)
4	1	reservoir	PEG400	2 (%)