1CFZ

HYDROGENASE MATURATING ENDOPEPTIDASE HYBD FROM E. COLI

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	298
Spacegroup name	P 32 1 2
Unit cell lengths	128.000, 128.000, 139.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.200
R-factor	0.224 *
Rwork	0.224
R-free	0.25900
Structure solution method	SIRAS
RMSD bond length	0.011
RMSD bond angle	0.021
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200	2.200 *
Rmerge	0.103 *	0.267 *
Total number of observations	125012 *
Number of reflections	62671
Completeness [%]	94.6	90.3 *
Redundancy	2.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	20 *	pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25 (mg/ml)
2	1	drop	MES-NaOH	10 (mM)
3	1	reservoir	MES-NaOH	100 (mM)
4	1	reservoir	ammonium sulfate	0.9 (M)
5	1	reservoir		1 (mM)