1CFW
GA-SUBSTITUTED DESULFOREDOXIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Temperature [K] | 293 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 42.200, 42.200, 72.300 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.700 - 1.900 |
R-factor | 0.179 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 0.026 |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.700 | |
High resolution limit [Å] | 1.950 | |
Rmerge | 0.118 * | 0.368 * |
Total number of observations | 46772 * | |
Number of reflections | 6148 | |
Completeness [%] | 98.2 | 93.9 * |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.6 * | pH 4.50 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 7 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 10 (mM) | |
3 | 1 | reservoir | ammonium sulfate |