1CFS
ANTI-P24 (HIV-1) FAB FRAGMENT CB41 COMPLEXED WITH AN EPITOPE-UNRELATED PEPTIDE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 288 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 85.320, 85.320, 136.770 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 72.000 - 2.750 |
| R-factor | 0.232 * |
| Rwork | 0.232 |
| R-free | 0.33200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.040 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CCP4 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.000 | |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.062 | |
| Number of reflections | 13769 | |
| <I/σ(I)> | 4.1 | |
| Completeness [%] | 100.0 | |
| Redundancy | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 15% POLYETHYLENE GLYCOL 4000, 0.1 M (NH4)2SO4, 0.2% ETHANOL, pH 6.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG4000 | 15 (%) | |
| 3 | 1 | reservoir | ammonium sulfate | 0.1 (M) | |
| 4 | 1 | reservoir | ethanol | 0.2 (%) | |
| 5 | 1 | reservoir | MES/NaOH | 0.1 (M) | |
| 6 | 1 | reservoir | 0.02 (%) |
