1CEZ

CRYSTAL STRUCTURE OF A T7 RNA POLYMERASE-T7 PROMOTER COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC
Spacegroup name	P 21 21 2
Unit cell lengths	220.100, 73.300, 80.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 2.400
R-factor	0.224
Rwork	0.224
R-free	0.27000
Structure solution method	MIRAS
RMSD bond length	0.010
RMSD bond angle	20.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((SCALA))
Phasing software	MLPHARE
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.080 *
Number of reflections	50077
<I/σ(I)>	11.9	1.9
Completeness [%]	96.7	97.3
Redundancy	2.9	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.7		pH 8.7

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	PEG8000	20 (%)
3	1	reservoir	ammonium sulfate	200 (mM)
4	1	reservoir	beta-n-octylglucopyranoside	0.25 (%)
5	1	reservoir	Tris-HCl	100 (mM)