1CES
CRYSTALS OF DEMETALLIZED CONCANAVALIN A SOAKED WITH ZINC HAVE A ZINC ION BOUND IN THE S1 SITE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS |
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1993-06-09 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 61.310, 86.200, 91.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DEMETALLIZED CONCANAVALIN A (PDB ENTRY 1APN) |
| RMSD bond length | 0.019 |
| RMSD bond angle | 27.800 * |
| Data reduction software | MADNES |
| Data scaling software | CCP4 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.700 |
| Rmerge | 0.085 |
| Total number of observations | 29303 * |
| Number of reflections | 12557 |
| Completeness [%] | 91.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5 | pH 5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1 (M) | |
| 3 | 1 | drop | sodium acetate | 50 (mM) | |
| 4 | 1 | drop | PEG6000 | 2.5 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) | |
| 6 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 5 (%) |
