1CEL
THE THREE-DIMENSIONAL CRYSTAL STRUCTURE OF THE CATALYTIC CORE OF CELLOBIOHYDROLASE I FROM TRICHODERMA REESEI
Experimental procedure
Spacegroup name | P 21 21 2 |
Unit cell lengths | 84.000, 86.200, 111.800 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 7.500 - 1.800 |
R-factor | 0.181 |
Rwork | 0.181 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.700 |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
High resolution limit [Å] | 1.810 * | 1.810 * |
Rmerge | 0.043 * | |
Total number of observations | 99089 * | |
Number of reflections | 65174 * | |
Completeness [%] | 95.5 * | 52 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 3.5 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5-10 (mg/ml) | 0.005ml |
2 | 1 | drop | glycine | 10 (mM) | |
3 | 1 | drop | PEG20000 | 10 (%) | 0.005ml |
4 | 1 | drop | calcium chloride | 50 (mM) | 0.001 ml |
5 | 1 | drop | o-iodobenzyl-1-thio-beta-D-cellobioside | 1 (mM) | |
6 | 1 | reservoir | sodium chloride | 0.2 (M) |