1CEI

STRUCTURE DETERMINATION OF THE COLICIN E7 IMMUNITY PROTEIN (IMME7) THAT BINDS SPECIFICALLY TO THE DNASE-TYPE COLICIN E7 AND INHIBITS ITS BACTERIOCIDAL ACTIVITY

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1994-11-21
Detector	RIGAKU RAXIS II
Spacegroup name	I 2 2 2
Unit cell lengths	75.140, 50.480, 45.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.187
Rwork	0.187
Structure solution method	MIR
RMSD bond length	0.010
RMSD bond angle	22.070 *
Data reduction software	MSC
Data scaling software	MSC
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.054	0.210
Total number of observations	31416 *
Number of reflections	7917
<I/σ(I)>		2.9
Completeness [%]	95.2	78.1
Redundancy	3.97	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		pH 8.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3 (mg/ml)
2	1	drop	ammonium phosphate	1.2 (M)
3	1	reservoir	ammonium phosphate	3.0 (M)