1CEF

CEFOTAXIME COMPLEXED WITH THE STREPTOMYCES R61 DD-PEPTIDASE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1989-11-29
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	51.300, 67.300, 102.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.040
R-factor	0.172
Rwork	0.172
R-free	0.24000
RMSD bond length	0.009
RMSD bond angle	1.800 *
Data reduction software	XENGEN
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
High resolution limit [Å]	2.040 *
Rmerge	0.057
Total number of observations	38280 *
Number of reflections	19106
Completeness [%]	67.0
Redundancy	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.8 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	20 (mg/ml)
2	1	drop	PEG8000	15 (%(w/v))
3	1	drop	phosphate	50 (mM)
4	1	reservoir	PEG8000	20 (%(w/v))