1CEC

A COMMON PROTEIN FOLD AND SIMILAR ACTIVE SITE IN TWO DISTINCT FAMILIES OF BETA-GLYCANASES

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Detector technology	IMAGE PLATE
Collection date	1993-11
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	51.400, 84.330, 87.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.150
R-factor	0.168
Rwork	0.168
R-free	0.21500
RMSD bond length	0.010
RMSD bond angle	23.620 *
Data reduction software	DENZO
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.150 *
Rmerge	0.086
Number of reflections	19891
Completeness [%]	90.7
Redundancy	7.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	24 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	reservoir	PEG2000	20 (%)
4	1	reservoir	calcium acetate	0.2 (M)
5	1	reservoir	Tris-HCl	0.1 (M)