1CEA
THE STRUCTURE OF THE NON-COVALENT COMPLEX OF RECOMBINANT KRINGLE 1 DOMAIN OF HUMAN PLASMINOGEN WITH EACA (EPSILON-AMINOCAPROIC ACID)
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 295 |
Detector technology | IMAGE PLATE |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 34.500, 51.600, 46.500 |
Unit cell angles | 90.00, 112.00, 90.00 |
Refinement procedure
Resolution | 7.000 - 2.060 |
R-factor | 0.168 |
RMSD bond length | 0.020 |
RMSD bond angle | 0.050 |
Data reduction software | R-AXIS |
Refinement software | PROLSQ |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 * | |
High resolution limit [Å] | 2.060 * | 2.060 * |
Rmerge | 0.076 | 0.128 * |
Total number of observations | 39205 * | |
Number of reflections | 9309 | |
<I/σ(I)> | 1 | |
Completeness [%] | 93.9 | 93.4 * |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6.5 * | seeding * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | citric acid | 1.6 (M) | |
2 | 1 | reservoir | sodium citrate | 1.4-1.5 (M) |