1CEA

THE STRUCTURE OF THE NON-COVALENT COMPLEX OF RECOMBINANT KRINGLE 1 DOMAIN OF HUMAN PLASMINOGEN WITH EACA (EPSILON-AMINOCAPROIC ACID)

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	295
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 1 21 1
Unit cell lengths	34.500, 51.600, 46.500
Unit cell angles	90.00, 112.00, 90.00

Refinement procedure

Resolution	7.000 - 2.060
R-factor	0.168
RMSD bond length	0.020
RMSD bond angle	0.050
Data reduction software	R-AXIS
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.060 *	2.060 *
Rmerge	0.076	0.128 *
Total number of observations	39205 *
Number of reflections	9309
<I/σ(I)>	1
Completeness [%]	93.9	93.4 *
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5 *		seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	citric acid	1.6 (M)
2	1	reservoir	sodium citrate	1.4-1.5 (M)