1CE9

HELIX CAPPING IN THE GCN4 LEUCINE ZIPPER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1998-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1
Unit cell lengths	28.304, 30.769, 42.884
Unit cell angles	75.41, 79.76, 90.08

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.214 *
Rwork	0.214
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2zta
RMSD bond length	0.004
RMSD bond angle	21.770 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.034	0.059
Total number of observations	23475 *
Number of reflections	11776
<I/σ(I)>	13.6	12
Completeness [%]	92.3	88.7
Redundancy	2	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		SEE REFERENCE, pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	20 (mg/ml)
2	1	reservoir	ammonium acetate	0.2 (M)
3	1	reservoir	sodium acetate	0.1 (M)
4	1	reservoir	PEG4000	20 (%)