1CE9
HELIX CAPPING IN THE GCN4 LEUCINE ZIPPER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-03 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.304, 30.769, 42.884 |
| Unit cell angles | 75.41, 79.76, 90.08 |
Refinement procedure
| Resolution | 15.000 - 1.800 |
| R-factor | 0.214 * |
| Rwork | 0.214 |
| R-free | 0.28300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zta |
| RMSD bond length | 0.004 |
| RMSD bond angle | 21.770 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.034 | 0.059 |
| Total number of observations | 23475 * | |
| Number of reflections | 11776 | |
| <I/σ(I)> | 13.6 | 12 |
| Completeness [%] | 92.3 | 88.7 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.6 | SEE REFERENCE, pH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 20 (mg/ml) | |
| 2 | 1 | reservoir | ammonium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%) |
