1CDZ

BRCT DOMAIN FROM DNA-REPAIR PROTEIN XRCC1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	300
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	100.800, 100.800, 72.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 3.200
Rwork	0.224
R-free	0.26600
Structure solution method	SIR
RMSD bond length	0.020
RMSD bond angle	2.100
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.072 *	0.286 *
Number of reflections	7526
<I/σ(I)>	6.2
Completeness [%]	99.5	100
Redundancy	4.7	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7		pH 7.0, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM FORMATE
2	1	2	SODIUM FORMATE

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.5 (mg/ml)
2	1	reservoir	sodium formate	4 (M)