1CDW
HUMAN TBP CORE DOMAIN COMPLEXED WITH DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 123 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 45.800, 78.000, 97.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.900 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | TBP2-DNA COMPLEX |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.680 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.227 |
| Total number of observations | 214715 * | |
| Number of reflections | 28256 | |
| Completeness [%] | 98.6 | 90.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.5 * | 4 * | used to seeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | hTBPc-DNA | 0.5 (mM) | |
| 10 | 1 | reservoir | glycerol | 8-12 (%(v/v)) | |
| 11 | 1 | reservoir | dithiothreitol | 20 (mM) | |
| 12 | 1 | reservoir | Tris-HCl | 40 (mM) | |
| 2 | 1 | drop | 200 (mM) | ||
| 3 | 1 | drop | ammonium acetate | 250 (mM) | |
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | 5 (mM) | ||
| 6 | 1 | drop | dithiothreitol | 10 (mM) | |
| 7 | 1 | drop | glycerol | 10 (%(v/v)) | |
| 8 | 1 | drop | ethylene glycol | 2 (%(v/v)) | |
| 9 | 1 | drop | Tris-HCl | 40 (mM) |
