1CDL

TARGET ENZYME RECOGNITION BY CALMODULIN: 2.4 ANGSTROMS STRUCTURE OF A CALMODULIN-PEPTIDE COMPLEX

Experimental procedure

Spacegroup name	P 21 21 2
Unit cell lengths	166.100, 104.900, 43.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.400 *
R-factor	0.22 *
Rwork	0.208
RMSD bond length	0.016 *
RMSD bond angle	3.460 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.400 *
Rmerge	0.068 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	CaM	24 (mg/ml)
2	1	drop	peptide	15 (mg/ml)
3	1	drop	PEG6000	25 (%(w/v))
4	1	drop	sodium azide	0.01 (%(w/v))
5	1	drop		5 (mM)
6	1	drop	Na acetate	50 (mM)
7	1	reservoir	PEG6000	25 (%(w/v))
8	1	reservoir	Na acetate	50 (mM)