1CDE
STRUCTURES OF APO AND COMPLEXED ESCHERICHIA COLI GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE
Experimental procedure
| Spacegroup name | P 1 |
| Unit cell lengths | 76.700, 72.600, 57.000 |
| Unit cell angles | 111.50, 82.80, 62.60 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.25 |
| Rwork | 0.250 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 3.500 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 * |
| High resolution limit [Å] | 2.500 * |
| Rmerge | 0.123 * |
| Number of reflections | 28188 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 30 (mg/ml) | |
| 2 | 1 | drop | GAR | ||
| 3 | 1 | drop | 5dTHF | ||
| 4 | 1 | reservoir | 154 (mM) | ||
| 5 | 1 | reservoir | Mops | 77 (mM) | |
| 6 | 1 | reservoir | PEG3400 | 19 (%(w/v)) | |
| 7 | 1 | reservoir | MPD | 4 (%(v/v)) |
