1CDD

STRUCTURES OF APO AND COMPLEXED ESCHERICHIA COLI GLYCINAMIDE RIBONUCLEOTIDE TRANSFORMYLASE

Experimental procedure

Spacegroup name	C 2 2 21
Unit cell lengths	140.900, 97.600, 102.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.800
R-factor	0.225
RMSD bond length	0.020
RMSD bond angle	4.100
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.074 *
Number of reflections	16623 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	Tris	50 (mM)
3	1	drop	dithiothreitol	1 (mM)
4	1	reservoir	Na+/K+ phosphate	0.8 (M)