1CD5
GLUCOSAMINE-6-PHOSPHATE DEAMINASE FROM E.COLI, T CONFORMER
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 277 |
| Collection date | 1998-01-15 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 129.810, 129.810, 139.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| Rwork | 0.207 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dea |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.300 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.400 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 * | 0.340 * |
| Number of reflections | 30597 | |
| <I/σ(I)> | 1.6 | |
| Completeness [%] | 98.0 | 98 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 20 * | sodium acetate, hepes, pH 7.0, VAPOR DIFFUSION, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM ACETATE | ||
| 2 | 1 | 1 | hepes |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | reservoir | sodium acetate | 1.75 (M) | |
| 3 | 1 | reservoir | HEPES | 100 (mM) |
