1CD3

PROCAPSID OF BACTERIOPHAGE PHIX174

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	277
Detector technology	IMAGE PLATE
Collection date	1997-02
Detector	FUJI
Spacegroup name	I 21 3
Unit cell lengths	774.000, 774.000, 774.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 3.500
R-factor	0.273 *
Rwork	0.275
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1PHX
RMSD bond length	0.016
RMSD bond angle	28.310 *
Data reduction software	DENZO
Phasing software	SnB
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	3.630
High resolution limit [Å]	3.500	3.500
Rmerge	0.217	0.360 *
Number of reflections	632194	25651 *
Completeness [%]	67.1	27.2 *
Redundancy	2.69	2.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	277 *	Dokland, T., (1998) Acta Cryst., D54, 878. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	MES	0.5 (M)	pH6.0
2	1	reservoir	ammonium sulfate	43-47 (%(v/v))
3	1	drop	protein	15-20 (mg/ml)