1CBR

CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID

Experimental procedure

Spacegroup name	P 43
Unit cell lengths	41.440, 41.440, 202.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.251
Rwork	0.251
R-free	0.32000
RMSD bond length	0.009
RMSD bond angle	1.560
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *
High resolution limit [Å]	2.900 *	2.900 *
Rmerge	0.086 *
Total number of observations	16898 *
Number of reflections	7118
Completeness [%]	94.2 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8 *	277 *	Bergfors, T., (1994) Acta Crystallogr.,Sect.D, 50, 370. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)
3	1	drop	beta-mercaptoethanol	1-2 (mM)
4	1	drop	beta-octylglucoside	0.1-0.2 (%)
5	1	reservoir	PEG8000	30-37.5 (%)
6	1	reservoir	HEPES	0.1 (M)
7	1	reservoir	sodium acetate	0.2 (M)	unbuffered
8	1	reservoir	isopropanol	5 (%)