1CBQ

CRYSTAL STRUCTURE OF CELLULAR RETINOIC-ACID-BINDING PROTEINS I AND II IN COMPLEX WITH ALL-TRANS-RETINOIC ACID AND A SYNTHETIC RETINOID

Experimental procedure

Spacegroup name	P 21 3
Unit cell lengths	81.040, 81.040, 81.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.21
Rwork	0.210
R-free	0.26400
RMSD bond length	0.012
RMSD bond angle	26.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.062 *	0.268 *
Total number of observations	46981 *
Number of reflections	9011
Completeness [%]	97.4 *	77.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	20 (mM)
2	1	reservoir	mPEG5000	10 (%(v/v))
3	1	reservoir	sodium citrate	0.1 (M)
4	1	reservoir	dimethyl sulphoxide	5 (%)