1CB8
CHONDROITINASE AC LYASE FROM FLAVOBACTERIUM HEPARINUM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 1996-10 |
Detector | ADSC |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 87.200, 87.200, 193.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 6.000 - 1.900 |
R-factor | 0.196 * |
Rwork | 0.195 |
R-free | 0.26000 |
Structure solution method | MIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 24.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.059 | 0.175 * |
Number of reflections | 60889 | |
<I/σ(I)> | 12 | 5.5 |
Completeness [%] | 91.3 | 78.8 * |
Redundancy | 3.1 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | unknown * | 6.5 | CRYSTALLIZATION CONDITIONS: PEG-MME 2K 17% AMMONIUM ACETATE 80MM PH 6.5 0.4M SODIUM ACETATE |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | mPEG2000 | 15-17 (%(w/v)) | |
2 | 1 | reservoir | ammonium acetate | 80 (mM) | |
3 | 1 | reservoir | sodium acetate | 400 (mM) |