1CB7
GLUTAMATE MUTASE FROM CLOSTRIDIUM COCHLEARIUM RECONSTITUTED WITH METHYL-COBALAMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.330, 113.140, 108.610 |
| Unit cell angles | 90.00, 95.62, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.000 |
| R-factor | 0.1597 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GLUTAMATE MUTASE RECONSTITUTED WITH CYANOCOBALAMIN |
| RMSD bond length | 0.005 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | X-PLOR |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.076 * | 0.189 * |
| Total number of observations | 258385 * | |
| Number of reflections | 100016 | |
| <I/σ(I)> | 5.6 | 1.3 |
| Completeness [%] | 96.1 | 96.2 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 4.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | sodium tartarate | ||
| 3 | 1 | 1 | METHYL COBALAMIN | ||
| 4 | 1 | 1 | CdCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | drop | (2S,4S)-45-fluoroglutamate | 5 (mM) | |
| 4 | 1 | drop | B12(cyano-or-methylcobalamin) | 2.5 (mg/ml) | |
| 5 | 1 | drop | Na cacodylate | 10 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 6 (%(w/v)) | |
| 7 | 1 | reservoir | DL-tartaric acid | 100 (M) |
