1CB6

STRUCTURE OF HUMAN APOLACTOFERRIN AT 2.0 A RESOLUTION.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS
Synchrotron site	SRS
Temperature [K]	295
Detector technology	FILM
Spacegroup name	P 21 21 21
Unit cell lengths	152.090, 94.580, 55.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.201
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1LGF (LACTOFERRIN)
RMSD bond length	0.003
RMSD bond angle	1.700 *
Data reduction software	MOSFLM
Phasing software	CCP4 ((ALMN))
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.000	2.120
High resolution limit [Å]	2.000	2.000
Rmerge	0.083	0.610
Number of reflections	53450
<I/σ(I)>	30.3	2.7
Completeness [%]	92.0	91
Redundancy	3.3	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7.8		pH 7.8

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	12 (mg/ml)
2	1	2	Tris-HCl	0.05 (M)
3	1	2	MPD	5 (%(v/v))
4	1	2	absolute ethanol	5 (%(v/v))