1CB5

HUMAN BLEOMYCIN HYDROLASE.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X26C
Synchrotron site	NSLS
Beamline	X26C
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	133.304, 171.494, 145.707
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 * - 2.590
R-factor	0.238 *
Rwork	0.237
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gcb
RMSD bond length	0.007
RMSD bond angle	21.200 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000 *	2.680
High resolution limit [Å]	2.590 *	2.590
Rmerge	0.072 *	0.431 *
Total number of observations	242494 *
Number of reflections	47154
<I/σ(I)>	18.8	3.5
Completeness [%]	91.6 *	94
Redundancy	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8	17 *	pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG10000	16-20 (%)
2	1	drop	protein	12 (mg/ml)
3	1	drop	Tris	25 (mM)
4	1	drop	glycerol	5 (%)
5	1	drop		140 (mM)
6	1	drop	dithiothreitol	1 (mM)