1CB0
STRUCTURE OF HUMAN 5'-DEOXY-5'-METHYLTHIOADENOSINE PHOSPHORYLASE AT 1.7 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 1997-11-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 120.760, 120.760, 44.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.700 |
| Rwork | 0.181 |
| R-free | 0.20200 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.800 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.045 * | 0.078 * |
| Total number of observations | 276082 * | |
| Number of reflections | 39479 | |
| <I/σ(I)> | 12.4 | 8.1 |
| Completeness [%] | 93.1 | 83.7 |
| Redundancy | 4.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | pH 7.4 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | ETHYLENE GLYCOL | ||
| 2 | 1 | 1 | PEG 6000 | ||
| 3 | 1 | 1 | TRIS BUFFER | ||
| 4 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 10 (%) | |
| 2 | 1 | drop | HEPES | 0.1 (M) | |
| 3 | 1 | drop | ethylene glycol | 8 (%) | |
| 4 | 1 | reservoir | PEG6000 | 12 (%(w/v)) | |
| 5 | 1 | reservoir | ethylene glycol | 25 (%(v/v)) | |
| 6 | 1 | reservoir | Tris-HCl | 200 (mM) | |
| 7 | 1 | reservoir | dithiothreitol | 2 (mM) |
