1CA9

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 IN COMPLEX WITH A PEPTIDE FROM TNF-R2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS IV
Spacegroup name	P 1 21 1
Unit cell lengths	83.000, 84.400, 100.700
Unit cell angles	90.00, 108.70, 90.00

Refinement procedure

Resolution	20.000 - 2.300
R-factor	0.234 *
Rwork	0.234
R-free	0.28900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ca4
RMSD bond length	0.007
RMSD bond angle	23.890 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Rmerge	0.048	0.279 *
Number of reflections	59650
Completeness [%]	99.0	97.9 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6 *	20 *	pH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	11 (%)
2	1	1	MES	0.1 (M)