1CA6
INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1996-09-01 |
Detector | RIGAKU RAXIS |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.470, 75.890, 35.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.200 |
R-factor | 0.197 * |
Rwork | 0.197 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1azp |
RMSD bond length | 0.014 * |
RMSD bond angle | 1.790 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | |
High resolution limit [Å] | 2.200 * | 2.200 * |
Rmerge | 0.058 | |
Number of reflections | 8013 | |
Completeness [%] | 84.4 | 72.9 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 6.5 | pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | Sac7d | 1.3 (mM) | |
2 | 1 | drop | DNA duplex | 1.3 (mM) | |
3 | 1 | drop | Tris-HCl | 2.5 (mM) | |
4 | 1 | drop | PEG400 | 2 (%) | |
5 | 1 | reservoir | PEG400 | 15 (%) |