1CA5

INTERCALATION SITE OF HYPERTHERMOPHILE CHROMOSOMAL PROTEIN SSO7D/SAC7D BOUND TO DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-08-15
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	49.780, 76.760, 35.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.19 *
Rwork	0.190
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1azp
RMSD bond length	0.008 *
RMSD bond angle	1.940 *
Phasing software	X-PLOR
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *	2.010
High resolution limit [Å]	2.200 *	2.200 *
Rmerge	0.630
Number of reflections	5268 *
Completeness [%]	73.8 *	60.9 *
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5		pH 6.5, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Sac7d	1.3 (mM)
2	1	drop	DNA duplex	1.3 (mM)
3	1	drop	Tris-HCl	2.5 (mM)
4	1	drop	PEG400	2 (%)
5	1	reservoir	PEG400	15 (%)