1CA4

STRUCTURE OF TNF RECEPTOR ASSOCIATED FACTOR 2 (TRAF2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Detector	ADSC
Spacegroup name	C 1 2 1
Unit cell lengths	134.920, 84.960, 124.070
Unit cell angles	90.00, 118.60, 90.00

Refinement procedure

Resolution	20.000 - 2.200
R-factor	0.209 *
Rwork	0.209
R-free	0.25100
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	24.600 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.200
Rmerge	0.043	0.107 *
Number of reflections	57967
Completeness [%]	92.7	98.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	20 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	PEG4000	11 (%)
2	1	reservoir	MES	0.1 (M)