1CA0
BOVINE CHYMOTRYPSIN COMPLEXED TO APPI
Experimental procedure
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1994-05 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.522, 181.488, 46.328 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.100 |
R-factor | 0.215 |
Rwork | 0.215 |
R-free | 0.32300 |
RMSD bond length | 0.007 |
RMSD bond angle | 26.210 * |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | X-PLOR (3.1) |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 2.100 |
Rmerge | 0.030 * |
Number of reflections | 33088 |
<I/σ(I)> | 39.87 |
Completeness [%] | 81.1 |
Redundancy | 3.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 4.5 | 4 * | pH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG6000 | 10-12.5 (%(w/v)) | |
2 | 1 | reservoir | sodium citrate | 100 (mM) | |
3 | 1 | reservoir | dimethylacetamide | 5 (%(v/v)) |