1C9Z
D232-CGTACG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 300 |
| Detector technology | AREA DETECTOR |
| Collection date | 1995-09-09 |
| Detector | UCSD MARK II |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 28.240, 28.240, 72.744 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.1961 |
| Rwork | 0.196 |
| R-free | 0.19610 |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 3.290 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | MADSYS |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.510 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.051 | 0.060 |
| Total number of observations | 5220 * | |
| Number of reflections | 744 * | |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 91.0 | 84 |
| Redundancy | 3.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 300 | MPD, SPERMINE, MAGNESIUM CHLORIDE, SODIUM CACODYLATE, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SPERMINE | ||
| 2 | 1 | 1 | MGCL2 | ||
| 3 | 1 | 1 | SODIUM CACODYLATE | ||
| 4 | 1 | 1 | MPD | ||
| 5 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | DNA single strand | 0.8 (mM) | |
| 2 | 1 | drop | sodium cacodylate | 15.8 (mM) | |
| 3 | 1 | drop | MPD | 2.6 (%) | |
| 4 | 1 | drop | spermine | 0.51 (mM) | |
| 5 | 1 | drop | 0.66 (mM) | ||
| 6 | 1 | drop | D232 | 0.37 (mM) | |
| 7 | 1 | reservoir | MPD | 20 (%) |
