1C9X

H119A VARIANT OF RIBONUCLEASE A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	275
Detector technology	AREA DETECTOR
Collection date	1998-05-28
Detector	SIEMENS HI-STAR
Spacegroup name	P 32 2 1
Unit cell lengths	64.530, 64.530, 65.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 1.800
RMSD bond length	0.011
RMSD bond angle	17.919 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	TNT
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.033	0.149
Total number of observations	68047 *
Number of reflections	23009
<I/σ(I)>	19
Completeness [%]	94.0	90
Redundancy	3.0 *	2.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	20 *	29% ammonium sulfate, 45% sodium chloride, 100mM sodium acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	sodium acetate	0.050 (M)
2	1	drop	protein	30 (mg/ml)
3	1	drop		22.5 (%(v/v)sat)
4	1	drop	ammonium sulfate	14.5 (%(v/v)sat)
5	1	reservoir	sodium acetate	0.10 (M)
6	1	reservoir		45 (%(v/v)sat)
7	1	reservoir	ammonium sulfate	29 (%(v/v)sat)