1C9T
COMPLEX OF BDELLASTASIN WITH BOVINE TRYPSIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 277 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04-17 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 95.220, 108.780, 95.710 |
Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
Resolution | 8.000 - 3.300 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.28700 |
RMSD bond length | 0.010 * |
RMSD bond angle | 1.561 * |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | AMoRE |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 3.420 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.110 | 0.290 |
Number of reflections | 23797 * | |
<I/σ(I)> | 4.3 | |
Completeness [%] | 81.2 | 77 |
Redundancy | 2.2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 21 * | 50MM MES, 2.0M AMMONIUM SULFATE, 10MM MAGNESIM CHLORIDE, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 * | 21 * | 50MM MES, 2.0M AMMONIUM SULFATE, 10MM MAGNESIM CHLORIDE, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | sodium chloride | 150 (mM) | |
3 | 1 | reservoir | ammonium sulfate | 2.2 (M) | |
4 | 1 | reservoir | magnesium chloride | 10 (mM) | |
5 | 1 | reservoir | MES | 50 (mM) |