1C9S
CRYSTAL STRUCTURE OF A COMPLEX OF TRP RNA-BINDING ATTENUATION PROTEIN WITH A 53-BASE SINGLE STRANDED RNA CONTAINING ELEVEN GAG TRIPLETS SEPARATED BY AU DINUCLEOTIDES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Collection date | 1997-10-25 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 150.970, 111.670, 138.680 |
Unit cell angles | 90.00, 117.77, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.900 |
R-factor | 0.188 * |
Rwork | 0.188 |
R-free | 0.23500 |
RMSD bond length | 0.010 * |
RMSD bond angle | 0.026 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.088 | 0.396 |
Number of reflections | 154404 * | |
<I/σ(I)> | 11.8 | |
Completeness [%] | 96.4 | 71.7 |
Redundancy | 3.3 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 2000 MONOMETHYL ETHER, TRIETHANOLAMINE, MGCL2, K-GLUTAMATE, K-PHOSPHATE, L- TRYPTOPHAN, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | drop | L-tryptophan | 7.5 (mM) | |
4 | 1 | reservoir | potassium glutamate | 0.2 (M) | |
5 | 1 | reservoir | triethanolamine | 50 (mM) | |
6 | 1 | reservoir | 10 (mM) | ||
7 | 1 | reservoir | mPEG2000 | 9-12 (%) |