1C9N
BACILLUS LENTUS SUBSTILISIN VARIANT (SER 87) K27R/V104Y/N123S/T274A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-08-12 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.250, 54.150, 84.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.163 |
| Rwork | 0.163 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.800 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 10.000 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.063 | 0.063 |
| Number of reflections | 27230 | |
| Completeness [%] | 77.0 | 77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.9 | SODIUM ACETATE, CALCIUM CHLORIDE, AMMONIUM SULFATE, pH 5.9, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 3 (mg/ml) | |
| 2 | 1 | drop | sodium acetate | 25 (mM) | |
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | ammonium sulfate | 14.5 (%(w/v)sat) | |
| 5 | 1 | reservoir | ammonium sulfate | 29 (%(w/v)sat) |
