1C9K
THE THREE DIMENSIONAL STRUCTURE OF ADENOSYLCOBINAMIDE KINASE/ ADENOSYLCOBINAMIDE PHOSPHATE GUALYLYLTRANSFERASE (COBU) COMPLEXED WITH GMP: EVIDENCE FOR A SUBSTRATE INDUCED TRANSFERASE ACTIVE SITE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 133 |
Detector technology | CCD |
Collection date | 1998-01-01 |
Detector | ADSC QUANTUM 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.380, 87.770, 101.640 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.200 |
R-factor | 0.191 |
RMSD bond length | 0.011 |
RMSD bond angle | 18.940 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.053 | 0.227 |
Number of reflections | 26435 | |
<I/σ(I)> | 27.5 | |
Completeness [%] | 97.3 | 94.9 |
Redundancy | 5.6 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 9 | 277 | 16% PEG 4000 100 MM CACL2 50 MM CHES, pH 9.0, BATCH |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | PEG4000 | 16 (%(w/v)) | |
2 | 1 | 1 | 100 (mM) | ||
3 | 1 | 1 | CHES | 50 (mM) |