1C9J
BACILLUS LENTUS SUBTILISIN K27R/N87S/V104Y/N123S/T274A VARIANT
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SEALED TUBE |
Source details | OTHER |
Detector technology | DIFFRACTOMETER |
Collection date | 1990-12-15 |
Detector | ENRAF-NONIUS CAD4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.000, 61.250, 75.100 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.148 |
RMSD bond length | 0.015 |
RMSD bond angle | 2.600 |
Data reduction software | CAD4 |
Data scaling software | CAD4 |
Refinement software | PROLSQ |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.066 * |
Number of reflections | 19999 |
Completeness [%] | 86.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 5.9 | SODIUM ACATEATE, CALCIUM CHLORIDE, AMMONIUM SULFATE, pH 5.9, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 3 (mg/ml) | |
2 | 1 | drop | sodium acetate | 25 (mM) | |
3 | 1 | drop | 5 (mM) | ||
4 | 1 | drop | ammonium sulfate | 14.5 (%(w/v)sat) | |
5 | 1 | reservoir | ammonium sulfate | 29 (%(w/v)sat) |