1C9I
PEPTIDE-IN-GROOVE INTERACTIONS LINK TARGET PROTEINS TO THE B-PROPELLER OF CLATHRIN
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ELLIOTT GX-13 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1999-04-01 |
Detector | MARRESEARCH |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.040, 131.275, 79.010 |
Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.900 |
R-factor | 0.227 |
Rwork | 0.227 |
R-free | 0.26800 * |
RMSD bond length | 0.014 |
RMSD bond angle | 1.900 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.080 |
High resolution limit [Å] | 2.700 | 2.900 |
Rmerge | 0.096 | 0.207 |
Number of reflections | 27038 | |
<I/σ(I)> | 12.3 | |
Completeness [%] | 97.3 | 94.6 |
Redundancy | 7.1 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 4000, KOAc, DTT, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | peptide | 10 (mM) | |
2 | 1 | drop | Tris-HCl | 100 (mM) | |
3 | 1 | reservoir | PEG4000 | 21 (%) | |
4 | 1 | reservoir | potassium acetate | 350 (mM) | |
5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
6 | 1 | reservoir | Tris-HCl | 100 (mM) |