1C9I
PEPTIDE-IN-GROOVE INTERACTIONS LINK TARGET PROTEINS TO THE B-PROPELLER OF CLATHRIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-13 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-04-01 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 137.040, 131.275, 79.010 |
| Unit cell angles | 90.00, 116.31, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.900 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26800 * |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.900 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 2.700 | 2.900 |
| Rmerge | 0.096 | 0.207 |
| Number of reflections | 27038 | |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 97.3 | 94.6 |
| Redundancy | 7.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | PEG 4000, KOAc, DTT, Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | peptide | 10 (mM) | |
| 2 | 1 | drop | Tris-HCl | 100 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 21 (%) | |
| 4 | 1 | reservoir | potassium acetate | 350 (mM) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
