1C9C
ASPARTATE AMINOTRANSFERASE COMPLEXED WITH C3-PYRIDOXAL-5'-PHOSPHATE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 156.980, 87.270, 79.730 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.202 * |
| Rwork | 0.202 |
| R-free | 0.27100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.077 | 0.220 |
| Total number of observations | 77327 * | |
| Number of reflections | 19863 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 91.0 | 90.6 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 20 * | ammonium sulfate, potassium phosphate, C3-pyridoxal-5p-phosphate, sodium azide, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 40 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 10 (mM) | |
| 3 | 1 | drop | PLP-amino acid | 0.010 (mM) | |
| 4 | 1 | drop | 0.3 (mM) | pH8.0 | |
| 5 | 1 | reservoir | ammonium sulfate | 35 (%sat) | |
| 6 | 1 | reservoir | potassium phosphate | 10 (mM) | |
| 7 | 1 | reservoir | 0.3 (mM) | pH8.0 |
