1C94

REVERSING THE SEQUENCE OF THE GCN4 LEUCINE ZIPPER DOES NOT AFFECT ITS FOLD.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	103
Detector technology	AREA DETECTOR
Collection date	1999-04-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	34.110, 34.090, 56.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.080
R-factor	0.1803
Rwork	0.180
R-free	0.28050
RMSD bond length	0.034
RMSD bond angle	3.102
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.080	2.000
Rmerge	0.035	0.110
Number of reflections	3740
Completeness [%]	87.2 *	80.7
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.8	295	drop contained 1:1 mixture of peptide and reservoir solution *
1	VAPOR DIFFUSION, HANGING DROP	4.8	295	drop contained 1:1 mixture of peptide and reservoir solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	peptide	2.5 (mg/ml)
2	1	reservoir	sodium chloride	0.2 (M)
3	1	reservoir	MPD	25 (%(v/v))
4	1	reservoir	sodium acetate	100 (mM)