1C93
Endo-Beta-N-Acetylglucosaminidase H, D130N/E132Q Double Mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-01-01 |
Detector | RIGAKU RAXIS IIC |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.300, 55.500, 46.700 |
Unit cell angles | 90.00, 104.50, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.100 |
R-factor | 0.205 * |
Rwork | 0.200 |
RMSD bond length | 0.013 |
RMSD bond angle | 25.730 * |
Phasing software | X-PLOR |
Refinement software | X-PLOR (3.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.093 * | |
Total number of observations | 39207 * | |
Number of reflections | 12430 | |
Completeness [%] | 96.5 | 92.3 |
Redundancy | 3.15 * |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 34% PEG1500, pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 0.003ml | |
2 | 1 | drop | reservoir | 0.006ml | |
3 | 1 | reservoir | PEG1500 | 34 (%) |